Points of interest near the route
- The Colonial Cafe
- Virginia Science and Technology Campus Library
- Taco Bell
- Chick-fil-A
- Martial Arts World
- Antonio's New York Pizza
- Art Institute of Washington-Dulles Campus
- University Center
- Center for Innovative Technology
- Galilee United Methodist Church
- Handicapped
- Popeyes
- Starbucks
- Cinnabon
- Food Court
- Smoothie King
Stops of Bus 321
In the order of the OpenStreetMap route relation.
- Innovation Center Metro Station - North Bus Bay421
- George Washington Blvd & Enterprise Hall52
- George Washington University & Research Place205
- George Washington Blvd & Research Pl
- George Washington Blvd & Braeton Bay Terrace
- George Washington Blvd & Innovation Hall209
- George Washington Blvd & Exploration Hall210
- Dulles Town Center Park & Ride25
- Innovation Center Metro Station - North Bus Bay421 (again)